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Literature summary for 2.7.4.1 extracted from
- Thermostable ATP regeneration system using polyphosphate kinase from Thermosynechococcus elongatus BP-1 for D-amino acid dipeptide synthesis (2007), J. Biosci. Bioeng., 103, 179-184.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | not activating | Thermosynechococcus vestitus |  |
| additional information | (NH4)2SO4 is not activating | Mycobacterium tuberculosis |
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | ATP supply for synthesis of D-amino acid dipeptides | Thermosynechococcus vestitus |
| biotechnology | ATP supply for synthesis of D-amino acid dipeptides | Mycobacterium tuberculosis |
| synthesis | the enzyme is used to establih a thermostable ATP regeneration system from polyphosphate substrate, this is then used for synthesis of D-Ala-D-Ala in a coupled system with the thermostable D-alanine-D-alanine ligase TmDdl from Thermotoga maritima, a useful biocatalyst for synthesizing D-amino acid dipeptides, method development and optimization, overview | Thermosynechococcus vestitus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His6-tagged Ppk in Escherichia coli strain BL21(DE3) | Thermosynechococcus vestitus |
| overexpression in Escherichia coli | Thermosynechococcus vestitus |
| overexpression in Escherichia coli | Mycobacterium tuberculosis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Thermosynechococcus vestitus | |
| Mg2+ | optimal above 0.5 mM | Thermosynechococcus vestitus | |
| Mg2+ | optimal above 0.5 mM | Mycobacterium tuberculosis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (phosphate)n | Thermosynechococcus vestitus | - |
ADP + (phosphate)n+1 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WHV9 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WHV9 | - |
- |
| Thermosynechococcus vestitus | - |
BP-1 | - |
| Thermosynechococcus vestitus | Q8DMA8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the recombinant His x6-tagged protein | Thermosynechococcus vestitus |
| of the recombinant His x6-tagged protein | Mycobacterium tuberculosis |
| recombinant His6-tagged Ppk 21fold from Escherichia coli strain BL21(DE3) by His-Trap chromatography | Thermosynechococcus vestitus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
- |
- |
of the purified protein | Thermosynechococcus vestitus |
| 0.317 | - |
purified recombinant His-tagged Ppk | Thermosynechococcus vestitus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + (phosphate)n | not (phosphate)3, (phosphate)4 as preferred phosphate donor | Thermosynechococcus vestitus | ATP + (phosphate)n-1 | - |
r | |
| ATP + (phosphate)n | - |
Mycobacterium tuberculosis | ADP + (phosphate)n+1 | - |
r | |
| ATP + (phosphate)n | - |
Thermosynechococcus vestitus | ADP + (phosphate)n+1 | - |
r | |
| ATP + (phosphate)n | - |
Mycobacterium tuberculosis H37Rv | ADP + (phosphate)n+1 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP:polyphosphate phosphotransferase | - |
Thermosynechococcus vestitus |
| ATP:polyphosphate phosphotransferase | - |
Mycobacterium tuberculosis |
| kinase, polyphosphate (phosphorylating) | - |
Thermosynechococcus vestitus |
| kinase, polyphosphate (phosphorylating) | - |
Mycobacterium tuberculosis |
| polyphosphate kinase | - |
Thermosynechococcus vestitus |
| polyphosphate kinase | - |
Mycobacterium tuberculosis |
| PPK | - |
Thermosynechococcus vestitus |
| PPK | - |
Mycobacterium tuberculosis |
| PPK2 | - |
Thermosynechococcus vestitus |
| PPK2B | - |
Thermosynechococcus vestitus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Thermosynechococcus vestitus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 75 | - |
Thermosynechococcus vestitus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
stable up to | Thermosynechococcus vestitus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Thermosynechococcus vestitus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Thermosynechococcus vestitus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Thermosynechococcus vestitus | |
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